Jessica Chastain Spanks Madonna On Stage in Prague

Madonna performs during her "Rebel Heart" tour
Kevin Mazur/Getty Images for Live Nation

Madonna performs during her "Rebel Heart" tour opener at Bell Centre on September 9, 2015 in Montreal, Canada.

Madonna's Rebel Heart spankfest continued during the tour's Saturday night (Nov. 7) stop in Prague. Only this time, Madge's special guest turned the tables and smacked the Queen's behind on stage.

Madonna's Rebel Heart Tour: $20 Million Earned & Counting 

Oscar-nominated actress Jessica Chastain joined Madonna during "Unapologetic Bitch," dancing to the reggae-dubstep jam and and spanking Madonna's ass when she crawled on all fours across the stage.

You can watch the antics in the fan-shot video below (spanking at :50) and see their respective Instagrams of the action beneath the YouTube clip.

The moment right before @madonna handed me a banana. The fkn QUEEN. #rebelheart #unapologeticbitch

A photo posted by Jessica Chastain (@chastainiac) on