Latifah also performed the song at Super Bowl XLIV four years ago.
The pregame show will also include the singing of the National Anthem, which this year will be performed by opera singer Renee Fleming, with Amber Zion performing in American Sign Language.
This year's halftime show will be headlined by Bruno Mars -- a winner at Sunday's GRAMMYs -- who will be joined by the Red Hot Chili Peppers.
More than 164 million viewers in the U.S. watched last year's Super Bowl, the most-watched television program in history.
The pregame and halftime shows are an NFL Network Production and will be executive produced by Ricky Kirshner.
The Super Bowl kicks off at 3:30 p.m. Sunday, live on Fox.
This article originally appeared on THR.com.