The three-stop tour will open Wednesday in an undisclosed location, with Gaga singing "Perfect Illusion" and "Million Reasons" from the album. The performance will be streamed live on Bud Light's Facebook page at a time to be announced, as will the Oct. 20 and 27 bar shows.
In a statement, Gaga said finding a way to bring people together is important to her.
"Which is why I love this dive bar tour so much, because I get to go perform these songs and really look my fans in the eyes when I'm singing to them and really try to have a more natural human experience," she said, instead of a "giant" space between her and an arena audience.
Lady Gaga Reveals 'Joanne' Track List
The 11-song album's title honors an aunt, her father's sister, who died at age 19; Joanne also is Gaga's middle name. "Perfect Illusion" was the first track out, in early September, with the album set for release on Oct. 21.
"This record was about me trying to understand my father, and his grief," she said. "It's also a record about me trying to understand myself and wondering where I'm going next in life. Because truly, the thing that I'm searching for the most is to feel connected to humanity, to feel connected as a person. So Joanne is like, you know, Lady Gaga if you erase all the fame."
The album, which Gaga said crosses over country, funk, pop and more, has its roots in her early days and "the music that lit up my heart when I first started writing" and singing in small venues.
The bar audiences will be made up of concert winners. For those who lost out, a sneak peek of select album tracks will be part of four TV spots airing nationally in October.