The rock legends lead for the first time since 2008, when "Rock N Roll Train" ruled for three weeks beginning that November.
Since then, the band charted five times before "Shot in the Dark," rising as high as No. 5 with "Play Ball" in November 2014.
AC/DC now has five total Mainstream Rock Songs No. 1s since the chart began in 1981. All have led since 1993, starting with "Big Gun," from the Last Action Hero soundtrack.
Concurrently, "Shot in the Dark" leaps 7-4 on the all-rock-format, audience-based Rock Airplay chart with 4.5 million audience impressions, up 10%, according to Nielsen Music/MRC Data.
The song is the lead single from Power Up, AC/DC's 17th studio album, due Friday, Nov. 13). The set is the follow-up to 2014's Rock or Bust, which reigned on Billboard's Hard Rock Albums chart for 10 weeks.